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SYVN1 Rabbit pAb (A2605)

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ABclonal:Western blot - SYVN1 Polyclonal Antibody (A2605)

Western blot analysis of extracts of various cell lines, using SYVN1 antibody (A2605) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review
ABclonal: review for SYVN1 Rabbit pAb(A2605)
Review
ABclonal: review for SYVN1 Rabbit pAb(A2605)

Overview

Product nameSYVN1 Rabbit pAb
Catalog No.A2605
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SYVN1 (NP_757385.1).
SequenceLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH
Gene ID84447
Swiss protQ86TM6
SynonymsSYVN1; DER3; HRD1
Calculated MW61kDa/67kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, HepG2, Mouse pancreas, Mouse lung, Mouse kidney
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein
Customer validation

(Mouse kidney)

WB(Homo sapiens,Rattus norvegicus, Mus musculus)

ABclonal:Western blot - SYVN1 Polyclonal Antibody (A2605) }

Western blot - SYVN1 Polyclonal Antibody (A2605)

Western blot analysis of extracts of various cell lines, using SYVN1 antibody (A2605) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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