Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SUPT4H1 Rabbit pAb (A7933)

Publication (1) Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - SUPT4H1 Rabbit pAb (A7933)

Western blot analysis of extracts of various cell lines, using SUPT4H1 Antibody (A7933) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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    Overview

    Product nameSUPT4H1 Rabbit pAb
    Catalog No.A7933
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene encodes the small subunit of DRB (5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human SUPT4H1 (NP_003159.1).
    SequenceMALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT
    Gene ID6827
    Swiss protP63272
    SynonymsSPT4; SPT4H; SUPT4H; Supt4a
    Calculated MW13kDa
    Observed MW14kDa

    Applications

    ReactivityHuman
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:2000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
    Application keyWestern blotting    
    Positive samplesMCF7, B cells
    Cellular locationNucleus
    Customer validation

    WB(Homo sapiens)

    SUPT4H1 Rabbit pAb images

    ABclonal:Western blot - SUPT4H1 Rabbit pAb (A7933)}

    Western blot - SUPT4H1 Rabbit pAb (A7933)

    Western blot analysis of extracts of various cell lines, using SUPT4H1 Antibody (A7933) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on SUPT4H1. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to SUPT4H1. (Distance between topics and target gene indicate popularity.) SUPT4H1

    * Data provided by citexs.com, for reference only.

    Publishing research using A7933? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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