Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SUPT4H1 Rabbit pAb (A7933)

Publication (1) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - SUPT4H1 Rabbit pAb (A7933)

Western blot analysis of extracts of various cell lines, using SUPT4H1 Antibody (A7933) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameSUPT4H1 Rabbit pAb
Catalog No.A7933
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes the small subunit of DRB (5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human SUPT4H1 (NP_003159.1).
SequenceMALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT
Gene ID6827
Swiss protP63272
SynonymsSUPT4H1; SPT4; SPT4H; SUPT4H; Supt4a
Calculated MW13kDa
Observed MW14kDa

Applications

ReactivityHuman
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMCF7, B cells
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

SUPT4H1 Rabbit pAb images

ABclonal:Western blot - SUPT4H1 Rabbit pAb (A7933)}

Western blot - SUPT4H1 Rabbit pAb (A7933)

Western blot analysis of extracts of various cell lines, using SUPT4H1 Antibody (A7933) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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