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STING / TMEM173 Rabbit pAb (A3575)

Reviews (4)Publications (2) Datasheet

ABclonal:Western blot - STING / TMEM173 Rabbit pAb (A3575)

Western blot analysis of extracts of various cell lines, using STING / TMEM173 antibody (A3575) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - STING / TMEM173 Rabbit pAb (A3575)

Western blot analysis of extracts of various cell lines, using STING / TMEM173 antibody (A3575) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - STING / TMEM173 Rabbit pAb (A3575)

Immunohistochemistry of paraffin-embedded rat pancreas using STING / TMEM173 antibody (A3575) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - STING / TMEM173 Rabbit pAb (A3575)

Immunofluorescence analysis of L929 cells using STING / TMEM173 antibody (A3575) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for STING / TMEM173 Rabbit pAb(A3575)
Review
ABclonal: review for STING / TMEM173 Rabbit pAb(A3575)
Review
ABclonal: review for STING / TMEM173 Rabbit pAb(A3575)
Review
ABclonal: review for STING / TMEM173 Rabbit pAb(A3575)

Overview

Product nameSTING / TMEM173 Rabbit pAb
Catalog No.A3575
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 170-379 of human STING / TMEM173 (NP_938023.1).
SequenceLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS
Gene ID340061
Swiss protQ86WV6
SynonymsTMEM173; ERIS; MITA; MPYS; NET23; SAVI; STING; hMITA; hSTING
Calculated MW42kDa
Observed MW35-40KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHT-29, THP-1, RAW264.7, Mouse spleen, Mouse thymus, Rat lung
Cellular locationCell membrane, Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Multi-pass membrane protein, perinuclear region
Customer validation

(HT-29 cells, THP1, Mouse MEF)

WB(Cynoglossus semilaevis)

IP(Mus musculus)

ABclonal:Western blot - STING / TMEM173 Rabbit pAb (A3575) }

Western blot - STING / TMEM173 Rabbit pAb (A3575)

Western blot analysis of extracts of various cell lines, using STING / TMEM173 antibody (A3575) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - STING / TMEM173 Rabbit pAb (A3575) }

Western blot - STING / TMEM173 Rabbit pAb (A3575)

Western blot analysis of extracts of various cell lines, using STING / TMEM173 antibody (A3575) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - STING / TMEM173 Rabbit pAb (A3575) }

Immunohistochemistry - STING / TMEM173 Rabbit pAb (A3575)

Immunohistochemistry of paraffin-embedded rat pancreas using STING / TMEM173 antibody (A3575) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - STING / TMEM173 Rabbit pAb (A3575) }

Immunofluorescence - STING / TMEM173 Rabbit pAb (A3575)

Immunofluorescence analysis of L929 cells using STING / TMEM173 antibody (A3575) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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