Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

STAU2 Rabbit pAb (A3413)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - STAU2 Rabbit pAb (A3413)

Western blot analysis of extracts of various cell lines, using STAU2 antibody (A3413) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - STAU2 Rabbit pAb (A3413)

Immunofluorescence analysis of U2OS cells using STAU2 antibody (A3413) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameSTAU2 Rabbit pAb
Catalog No.A3413
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Staufen homolog 2 is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. Staufen homolog 2 shares 48.5% and 59.9% similarity with drosophila and human staufen, respectively. The exact function of Staufen homolog 2 is not known, but since it contains 3 copies of conserved dsRNA binding domain, it could be involved in double-stranded RNA binding events. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human STAU2 (NP_001157852.1).
SequencePEYGQGMNPISRLAQIQQAKKEKEPDYVLLSERGMPRRREFVMQVKVGNEVATGTGPNKKIAKKNAAEAMLLQLGYKASTNLQDQLEKTGENKGWSGPKPG
Gene ID27067
Swiss protQ9NUL3
SynonymsSTAU2; 39K2; 39K3
Calculated MW11kDa/34kDa/43kDa/52kDa/55kDa/59kDa/62kDa
Observed MW52kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesMouse heart, Mouse kidney, Rat heart, Rat brain
Cellular locationCytoplasm, Endoplasmic reticulum, Nucleus, nucleolus

Research Area

STAU2 Rabbit pAb images

ABclonal:Western blot - STAU2 Rabbit pAb (A3413)}

Western blot - STAU2 Rabbit pAb (A3413)

Western blot analysis of extracts of various cell lines, using STAU2 antibody (A3413) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - STAU2 Rabbit pAb (A3413)}

Immunofluorescence - STAU2 Rabbit pAb (A3413)

Immunofluorescence analysis of U2OS cells using STAU2 antibody (A3413) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on STAU2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to STAU2. (Distance between topics and target gene indicate popularity.) STAU2

* Data provided by citexs.com, for reference only.

Publishing research using A3413? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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