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STAT1 Polyclonal Antibody (A0027)

Review (1)Publication (6) Datasheet

ABclonal:Western blot - STAT1 Polyclonal Antibody (A0027)
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A0027)
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A0027)
Review
ABclonal: review for STAT1 Polyclonal Antibody(A0027)

Overview

Product nameSTAT1 Polyclonal Antibody
Catalog No.A0027
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 513-712 of human STAT1 (NP_644671.1).
SequenceGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEV
Gene ID6772
Swiss protP42224
SynonymsCANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; STAT1 alpha; STAT1
Calculated MW83kDa/87kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesPC-3, HeLa, HepG2, A-549, MCF7, K-562, Mouse spleen
Cellular locationCytoplasm, Nucleus
Customer validation

WB(African green monkey, Human, Mouse)

Co-IP(Mouse)

ABclonal:Western blot - STAT1 Polyclonal Antibody (A0027) }

Western blot - STAT1 Polyclonal Antibody (A0027)

Western blot analysis of extracts of various cell lines, using STAT1 antibody (A0027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A0027) }

Immunohistochemistry - STAT1 Polyclonal Antibody (A0027)

Immunohistochemistry of paraffin-embedded rat brain using STAT1 antibody (A0027) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A0027) }

Immunohistochemistry - STAT1 Polyclonal Antibody (A0027)

Immunohistochemistry of paraffin-embedded mouse brain using STAT1 antibody (A0027) at dilution of 1:100 (40x lens).

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