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SREBP1 Rabbit pAb (A15586)

Publications (8) Datasheet

ABclonal:Western blot - SREBP1 Rabbit pAb (A15586)

Western blot analysis of extracts of various cell lines, using SREBP1 antibody (A15586) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - SREBP1 Rabbit pAb (A15586)

Immunohistochemistry of paraffin-embedded Human liver cancer using SREBP1 antibody (A15586) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SREBP1 Rabbit pAb (A15586)

Immunohistochemistry of paraffin-embedded Mouse liver using SREBP1 antibody (A15586) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SREBP1 Rabbit pAb (A15586)

Immunohistochemistry of paraffin-embedded Rat liver using SREBP1 antibody (A15586) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameSREBP1 Rabbit pAb
Catalog No.A15586
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SREBF1 (NP_001005291.1).
SequenceMDEPPFSEAALEQALGEPCDLDAALLTDIEGEVGAGRGRANGLDAPRAGADRGAMDCTFEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPG
Gene ID6720
Swiss protP36956
SynonymsSREBF1; SREBP-1c; SREBP1; SREBP1a; bHLHd1
Calculated MW45kDa/48kDa/111kDa/113kDa/121kDa/124kDa
Observed MW68KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549, MCF7, U-87MG, Mouse liver
Cellular locationCOPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus
Customer validation

WB(Mus musculus, Sus scrofa, Gallus gallus)

ABclonal:Western blot - SREBP1 Rabbit pAb (A15586) }

Western blot - SREBP1 Rabbit pAb (A15586)

Western blot analysis of extracts of various cell lines, using SREBP1 antibody (A15586) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - SREBP1 Rabbit pAb (A15586) }

Immunohistochemistry - SREBP1 Rabbit pAb (A15586)

Immunohistochemistry of paraffin-embedded Human liver cancer using SREBP1 antibody (A15586) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SREBP1 Rabbit pAb (A15586) }

Immunohistochemistry - SREBP1 Rabbit pAb (A15586)

Immunohistochemistry of paraffin-embedded Mouse liver using SREBP1 antibody (A15586) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SREBP1 Rabbit pAb (A15586) }

Immunohistochemistry - SREBP1 Rabbit pAb (A15586)

Immunohistochemistry of paraffin-embedded Rat liver using SREBP1 antibody (A15586) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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