Reviews (8)Publications (33) Datasheet
Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A7758) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens).
Confocal immunofluorescence analysis of Hela cells using SQSTM1/p62 Polyclonal Antibody (A7758) at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2 OS cells using SQSTM1/p62 Polyclonal Antibody (A7758) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SQSTM1/p62 antibody (A7758). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A7758) at a dilition of 1:1000.
| Product name | SQSTM1/p62 Rabbit pAb |
|---|---|
| Catalog No. | A7758 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_001135770.1). |
|---|---|
| Sequence | MAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL |
| Gene ID | 8878 |
| Swiss prot | Q13501 |
| Synonyms | SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B |
| Calculated MW | 38kDa/47kDa |
| Observed MW | 62KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | PC-12, L929, C2C12, HeLa |
| Cellular location | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome |
| Customer validation | (U-87MG cells, Human lung cancer cell, PANC-1, Squamous cell carcinoma, A549, Mouse cells, HepG2, Human Isikawa ) WB(Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio, Bombyx mori, Spodoptera frugiperda, Sus scrofa) IP(Homo sapiens) IHC(Mus musculus) WB(Mus musculus) IHC(Mus musculus) IP(Homo sapiens) |
* For research use only. Not for therapeutic or diagnostic purposes.
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