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SOD2 Rabbit pAb (A1340)

Review (1)Publications (17) Datasheet

ABclonal:Western blot - SOD2 Rabbit pAb (A1340)
ABclonal:Western blot - SOD2 Rabbit pAb (A1340)
ABclonal:Immunohistochemistry - SOD2 Rabbit pAb (A1340)
ABclonal:Immunohistochemistry - SOD2 Rabbit pAb (A1340)
ABclonal:Immunohistochemistry - SOD2 Rabbit pAb (A1340)
Review
ABclonal: review for SOD2 Rabbit pAb(A1340)

Overview

Product nameSOD2 Rabbit pAb
Catalog No.A1340
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
SequenceKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWTNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYMACKK
Gene ID6648
Swiss protP04179
SynonymsIPO-B; IPOB; MNSOD; MVCD6; Mn-SOD; SOD2
Calculated MW18kDa/19kDa/20kDa/24kDa
Observed MW25KDa/22KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, HepG2, A-549, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain, Rat heart
Cellular locationMitochondrion matrix
Customer validation

(NPCs)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Bos taurus)

Co-IP(Mus musculus)

ABclonal:Western blot - SOD2 Rabbit pAb (A1340) }

Western blot - SOD2 Rabbit pAb (A1340)

Western blot analysis of extracts of various cell lines, using SOD2 antibody (A1340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - SOD2 Rabbit pAb (A1340) }

Western blot - SOD2 Rabbit pAb (A1340)

Western blot analysis of extracts from normal (control) and SOD2 Rabbit pAb knockout (KO) 293T cells, using SOD2 Rabbit pAb antibody (A1340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - SOD2 Rabbit pAb (A1340) }

Immunohistochemistry - SOD2 Rabbit pAb (A1340)

Immunohistochemistry of paraffin-embedded rat testis using SOD2 Rabbit pAb (A1340) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SOD2 Rabbit pAb (A1340) }

Immunohistochemistry - SOD2 Rabbit pAb (A1340)

Immunohistochemistry of paraffin-embedded human colon using SOD2 Rabbit pAb (A1340) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SOD2 Rabbit pAb (A1340) }

Immunohistochemistry - SOD2 Rabbit pAb (A1340)

Immunohistochemistry of paraffin-embedded mouse kidney using SOD2 Rabbit pAb (A1340) at dilution of 1:100 (40x lens).

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