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SOD2 Polyclonal Antibody (A1340)

Review (1)Publications (8) Datasheet

ABclonal:Western blot - SOD2 Polyclonal Antibody (A1340)
ABclonal:Immunohistochemistry - SOD2 Polyclonal Antibody (A1340)
ABclonal:Immunohistochemistry - SOD2 Polyclonal Antibody (A1340)
ABclonal:Immunofluorescence - SOD2 Polyclonal Antibody (A1340)
ABclonal:Immunofluorescence - SOD2 Polyclonal Antibody (A1340)

Overview

Product nameSOD2 Polyclonal Antibody
Catalog No.A1340
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
SequenceKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWTNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYMACKK
Gene ID6648
Swiss protP04179
SynonymsIPO-B; IPOB; Mn-SOD; MNSOD; MVCD6
Calculated MW18kDa/19kDa/20kDa/24kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, HepG2, DU145, U-251MG, Mouse kidney, Mouse liver, Mouse heart, Mouse skeletal muscle
Cellular locationMitochondrion matrix
Customer validation

WB(NPCs, Mouse, Human, Rat, Pig)

ABclonal:Western blot - SOD2 Polyclonal Antibody (A1340) }

Western blot - SOD2 Polyclonal Antibody (A1340)

Western blot analysis of extracts of various cell lines, using SOD2 antibody (A1340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - SOD2 Polyclonal Antibody (A1340) }

Immunohistochemistry - SOD2 Polyclonal Antibody (A1340)

Immunohistochemistry of paraffin-embedded rat heart using SOD2 antibody (A1340) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SOD2 Polyclonal Antibody (A1340) }

Immunohistochemistry - SOD2 Polyclonal Antibody (A1340)

Immunohistochemistry of paraffin-embedded human oophoroma using SOD2 antibody (A1340) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - SOD2 Polyclonal Antibody (A1340) }

Immunofluorescence - SOD2 Polyclonal Antibody (A1340)

Immunofluorescence analysis of HeLa cells using SOD2 antibody (A1340). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SOD2 Polyclonal Antibody (A1340) }

Immunofluorescence - SOD2 Polyclonal Antibody (A1340)

Immunofluorescence analysis of PC-12 cells using SOD2 antibody (A1340) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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