Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SOCS4 Rabbit pAb (A8003)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - SOCS4 Rabbit pAb (A8003)

Western blot analysis of various lysates, using SOCS4 Rabbit pAb (A8003) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name SOCS4 Rabbit pAb
Catalog No. A8003
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS), also known as STAT-induced STAT inhibitor (SSI), protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 340-440 of human SOCS4 (NP_543143.1).
Sequence SKVRVLRIDAPEQQC
Gene ID 122809
Swiss prot Q8WXH5
Synonyms SOCS7; SOCS4
Calculated MW 51kDa
Observed MW 51kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples K-562, HeLa, NIH/3T3
Cellular location

Documents

Certificate of Compliance

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Lot number

Research Area

SOCS4 Rabbit pAb images

ABclonal:Western blot - SOCS4 Rabbit pAb (A8003)}

Western blot - SOCS4 Rabbit pAb (A8003)

Western blot analysis of various lysates, using SOCS4 Rabbit pAb (A8003) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SOCS4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SOCS4. (Distance between topics and target gene indicate popularity.) SOCS4

* Data provided by citexs.com, for reference only.

Publishing research using A8003? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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