Products > Catalog Antibodies > Polyclonal Antibodies

SOCS3 Rabbit pAb (A0694)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - SOCS3 Rabbit pAb (A0694)

Western blot analysis of extracts of RAW264.7 cells, using SOCS3 antibody (A0694) at 1:500 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - SOCS3 Rabbit pAb (A0694)

Immunohistochemistry of paraffin-embedded mouse heart using SOCS3 Rabbit pAb (A0694) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - SOCS3 Rabbit pAb (A0694)

Immunohistochemistry of paraffin-embedded mouse spleen using SOCS3 Rabbit pAb (A0694) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - SOCS3 Rabbit pAb (A0694)

Immunohistochemistry of paraffin-embedded rat heart using SOCS3 Rabbit pAb (A0694) at dilution of 1:50 (40x lens).

Review
ABclonal: review for SOCS3 Rabbit pAb(A0694)
Review
ABclonal: review for SOCS3 Rabbit pAb(A0694)

Overview

Product nameSOCS3 Rabbit pAb
Catalog No.A0694
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human SOCS3 (NP_003946.3).
SequenceGSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPPTEPSSEVPEQPSAQPLPGSPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYDAPL
Gene ID9021
Swiss protO14543
SynonymsSOCS3; ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3
Calculated MW24kDa
Observed MW30KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRAW264.7
Cellular location
Customer validation

(PK15, Mouse liver)

WB(Homo sapiens)

ABclonal:Western blot - SOCS3 Rabbit pAb (A0694) }

Western blot - SOCS3 Rabbit pAb (A0694)

Western blot analysis of extracts of RAW264.7 cells, using SOCS3 antibody (A0694) at 1:500 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - SOCS3 Rabbit pAb (A0694) }

Immunohistochemistry - SOCS3 Rabbit pAb (A0694)

Immunohistochemistry of paraffin-embedded mouse heart using SOCS3 Rabbit pAb (A0694) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - SOCS3 Rabbit pAb (A0694) }

Immunohistochemistry - SOCS3 Rabbit pAb (A0694)

Immunohistochemistry of paraffin-embedded mouse spleen using SOCS3 Rabbit pAb (A0694) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - SOCS3 Rabbit pAb (A0694) }

Immunohistochemistry - SOCS3 Rabbit pAb (A0694)

Immunohistochemistry of paraffin-embedded rat heart using SOCS3 Rabbit pAb (A0694) at dilution of 1:50 (40x lens).

Submit an review for A0694

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A0694 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A0694? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order