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SLUG Polyclonal Antibody (A1057)

Reviews (4)Publications (2) Datasheet

ABclonal:Western blot - SLUG Polyclonal Antibody (A1057)
ABclonal:Immunohistochemistry - SLUG Polyclonal Antibody (A1057)
ABclonal:Immunohistochemistry - SLUG Polyclonal Antibody (A1057)
ABclonal:Immunohistochemistry - SLUG Polyclonal Antibody (A1057)
ABclonal:Immunofluorescence - SLUG Polyclonal Antibody (A1057)
Review
ABclonal: review for SLUG Polyclonal Antibody(A1057)
Review
ABclonal: review for SLUG Polyclonal Antibody(A1057)
Review
ABclonal: review for SLUG Polyclonal Antibody(A1057)
Review
ABclonal: review for SLUG Polyclonal Antibody(A1057)

Overview

Product nameSLUG Polyclonal Antibody
Catalog No.A1057
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 89-268 of human SLUG (NP_003059.1).
SequencePPPSDTSSKDHSGSESPISDEEERLQSKLSDPHAIEAEKFQCNLCNKTYSTFSGLAKHKQLHCDAQSRKSFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGCCVAH
Gene ID6591
Swiss protO43623
SynonymsSNAI2; SLUG; SLUGH; SLUGH1; SNAIL2; WS2D; zinc finger protein SNAI2
Calculated MW29kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart, Rat heart
Cellular locationCytoplasm, Nucleus
Customer validation

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WB(human)

IF(Human)

ABclonal:Western blot - SLUG Polyclonal Antibody (A1057) }

Western blot - SLUG Polyclonal Antibody (A1057)

Western blot analysis of extracts of various cell lines, using SLUG antibody (A1057) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - SLUG Polyclonal Antibody (A1057) }

Immunohistochemistry - SLUG Polyclonal Antibody (A1057)

Immunohistochemistry of paraffin-embedded rat brain using SLUG Antibody (A1057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SLUG Polyclonal Antibody (A1057) }

Immunohistochemistry - SLUG Polyclonal Antibody (A1057)

Immunohistochemistry of paraffin-embedded rat spleen using SLUG Antibody (A1057) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SLUG Polyclonal Antibody (A1057) }

Immunohistochemistry - SLUG Polyclonal Antibody (A1057)

Immunohistochemistry of paraffin-embedded human liver cancer using SLUG Antibody (A1057) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - SLUG Polyclonal Antibody (A1057) }

Immunofluorescence - SLUG Polyclonal Antibody (A1057)

Immunofluorescence analysis of A549 cells using SLUG antibody (A1057). Blue: DAPI for nuclear staining.

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