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SNAIL Polyclonal Antibody (A5243)

Reviews (2)Publications (6) Datasheet

ABclonal:Western blot - SNAIL Polyclonal Antibody (A5243)
ABclonal:Immunohistochemistry - SNAIL Polyclonal Antibody (A5243)
ABclonal:Immunohistochemistry - SNAIL Polyclonal Antibody (A5243)
ABclonal:Immunofluorescence - SNAIL Polyclonal Antibody (A5243)
Review
ABclonal: review for SNAIL Polyclonal Antibody(A5243)
Review
ABclonal: review for SNAIL Polyclonal Antibody(A5243)

Overview

Product nameSNAIL Polyclonal Antibody
Catalog No.A5243
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SNAIL (NP_005976.2).
SequenceMPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGS
Gene ID6615
Swiss protO95863
SynonymsSNAI1; SLUGH2; SNA; SNAH; SNAIL; SNAIL1; dJ710H13.1; zinc finger protein SNAI1
Calculated MW29kDa
Observed MW29kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, K-562, NIH/3T3, Mouse lung, Mouse heart, Rat lung
Cellular locationCytoplasm, Nucleus
Customer validation

(, )

WB(Human, Mouse)

ICC(Human)

IF(Human)

IF(Human)

ABclonal:Western blot - SNAIL Polyclonal Antibody (A5243) }

Western blot - SNAIL Polyclonal Antibody (A5243)

Western blot analysis of extracts of various cell lines, using SNAIL antibody (A5243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - SNAIL Polyclonal Antibody (A5243) }

Immunohistochemistry - SNAIL Polyclonal Antibody (A5243)

Immunohistochemistry of paraffin-embedded human breast using SNAIL antibody (A5243) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SNAIL Polyclonal Antibody (A5243) }

Immunohistochemistry - SNAIL Polyclonal Antibody (A5243)

Immunohistochemistry of paraffin-embedded human colon using SNAIL antibody (A5243) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - SNAIL Polyclonal Antibody (A5243) }

Immunofluorescence - SNAIL Polyclonal Antibody (A5243)

Immunofluorescence analysis of U2OS cells using SNAIL antibody (A5243) at dilution of 1:100. Blue: DAPI for nuclear staining.

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