Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

SMYD1 Mouse mAb (A9924)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - SMYD1 Mouse mAb (A9924)

Western blot analysis of various lysates using SMYD1 Mouse mAb (A9924) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name SMYD1 Mouse mAb
Catalog No. A9924
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable histone-lysine N-methyltransferase activity. Involved in positive regulation of myoblast differentiation and positive regulation of myotube differentiation. Predicted to be located in cytoplasm. Predicted to be active in nucleus.

Immunogen information

Immunogen Recombinant protein of human SMYD1
Sequence Email for sequence
Gene ID 150572
Swiss prot Q8NB12
Synonyms BOP; KMT3D; ZMYND18; ZMYND22; SMYD1
Calculated MW 57kDa
Observed MW 56kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse skeletal muscle, Mouse heart, Rat heart, Rat skeletal muscle
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

SMYD1 Mouse mAb images

ABclonal:Western blot - SMYD1 Mouse mAb (A9924)}

Western blot - SMYD1 Mouse mAb (A9924)

Western blot analysis of various lysates using SMYD1 Mouse mAb (A9924) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SMYD1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMYD1. (Distance between topics and target gene indicate popularity.) SMYD1

* Data provided by citexs.com, for reference only.

Publishing research using A9924? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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