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SMO Rabbit pAb (A3274)

Review (1)Publications (9) Datasheet

ABclonal:Western blot - SMO Polyclonal Antibody (A3274)

Western blot analysis of extracts of various cell lines, using SMO antibody (A3274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SMO Polyclonal Antibody (A3274)

Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (A3274) (40x lens).

ABclonal:Immunofluorescence - SMO Polyclonal Antibody (A3274)

Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SMO Polyclonal Antibody (A3274)

Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for SMO Rabbit pAb(A3274)

Overview

Product nameSMO Rabbit pAb
Catalog No.A3274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 554-787 of human SMO (NP_005622.1).
SequenceGQSDDEPKRIKKSKMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPVPPEEQANLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF
Gene ID6608
Swiss protQ99835
SynonymsGx; CRJS; SMOH; FZD11; SMO
Calculated MW86kDa
Observed MW86kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, Mouse kidney
Cellular locationCell projection, Membrane, Multi-pass membrane protein, cilium
Customer validation

(Mouse liver)

WB(Mus musculus, Drosophila, Rattus norvegicus, Homo sapiens)

IP(Drosophila melanogaster)

WB(Drosophila melanogaster)

ABclonal:Western blot - SMO Polyclonal Antibody (A3274) }

Western blot - SMO Polyclonal Antibody (A3274)

Western blot analysis of extracts of various cell lines, using SMO antibody (A3274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - SMO Polyclonal Antibody (A3274) }

Immunohistochemistry - SMO Polyclonal Antibody (A3274)

Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (A3274) (40x lens).
ABclonal:Immunofluorescence - SMO Polyclonal Antibody (A3274) }

Immunofluorescence - SMO Polyclonal Antibody (A3274)

Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SMO Polyclonal Antibody (A3274) }

Immunofluorescence - SMO Polyclonal Antibody (A3274)

Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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