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SMO Rabbit pAb (A3274)

Review (1)Publications (10) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse

ABclonal:Western blot - SMO Rabbit pAb (A3274)

Western blot analysis of extracts of various cell lines, using SMO antibody (A3274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

ABclonal:Western blot - SMO Rabbit pAb (A3274)

Western blot analysis of extracts of Mouse brain, using SMO antibody (A3274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - SMO Rabbit pAb (A3274)

Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SMO Rabbit pAb (A3274)

Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for SMO Rabbit pAb(A3274)

Overview

Product nameSMO Rabbit pAb
Catalog No.A3274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 54-219 of human SMO (NP_005622.1).
SequenceVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQN
Gene ID6608
Swiss protQ99835
SynonymsGx; CRJS; SMOH; FZD11; SMO
Calculated MW86kDa
Observed MW86KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293T, Mouse brain
Cellular locationCell projection, Membrane, Multi-pass membrane protein, cilium
Customer validation

(Mouse liver)

WB(Mus musculus, Drosophila, Rattus norvegicus, Homo sapiens)

IP(Drosophila melanogaster)

WB(Drosophila melanogaster)

ABclonal:Western blot - SMO Rabbit pAb (A3274)}

Western blot - SMO Rabbit pAb (A3274)

Western blot analysis of extracts of various cell lines, using SMO antibody (A3274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.
ABclonal:Western blot - SMO Rabbit pAb (A3274)}

Western blot - SMO Rabbit pAb (A3274)

Western blot analysis of extracts of Mouse brain, using SMO antibody (A3274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - SMO Rabbit pAb (A3274)}

Immunofluorescence - SMO Rabbit pAb (A3274)

Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SMO Rabbit pAb (A3274)}

Immunofluorescence - SMO Rabbit pAb (A3274)

Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (A3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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