Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SMARCA5/SNF2H Rabbit pAb (A2000)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - SMARCA5/SNF2H Rabbit pAb (A2000)

Western blot analysis of extracts of various cell lines, using SMARCA5/SNF2H antibody (A2000) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.

You may also interested in:

Overview

Product name SMARCA5/SNF2H Rabbit pAb
Catalog No. A2000
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5/SNF2H (NP_003592.3).
Sequence ELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGDYRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLGKTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWDVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLLNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADV
Gene ID 8467
Swiss prot O60264
Synonyms ISWI; SNF2H; hISWI; hSNF2H; WCRF135; SMARCA5/SNF2H
Calculated MW 122kDa
Observed MW 125kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples PC-3, H460, 293T, HepG2, SH-SY5Y, Mouse spleen, Mouse brain, Mouse testis
Cellular location Nucleus
Customer validation

WB (Mus musculus, Homo sapiens)

IF (Homo sapiens)

IP (Homo sapiens)

Northern blot (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

SMARCA5/SNF2H Rabbit pAb images

ABclonal:Western blot - SMARCA5/SNF2H Rabbit pAb (A2000)}

Western blot - SMARCA5/SNF2H Rabbit pAb (A2000)

Western blot analysis of extracts of various cell lines, using SMARCA5/SNF2H antibody (A2000) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.

Inquire About This Product

Submit your question about A2000 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on SMARCA5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SMARCA5. (Distance between topics and target gene indicate popularity.) SMARCA5

* Data provided by citexs.com, for reference only.

Publishing research using A2000? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order