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SMARCA5/SNF2H Rabbit pAb (A2000)

Publications (2) Datasheet

ABclonal:Western blot - SMARCA5/SNF2H Rabbit pAb (A2000)

Western blot analysis of extracts of various cell lines, using SMARCA5/SNF2H antibody (A2000) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.

ABclonal:Immunofluorescence - SMARCA5/SNF2H Rabbit pAb (A2000)

Immunofluorescence analysis of HeLa cells using SMARCA5/SNF2H antibody (A2000). Blue: DAPI for nuclear staining.

Overview

Product nameSMARCA5/SNF2H Rabbit pAb
Catalog No.A2000
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5/SNF2H (NP_003592.3).
SequenceELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGDYRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLGKTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWDVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLLNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADV
Gene ID8467
Swiss protO60264
SynonymsSMARCA5; ISWI; SNF2H; WCRF135; hISWI; hSNF2H
Calculated MW121kDa
Observed MW125kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesPC-3, H460, 293T, HepG2, SH-SY5Y, Mouse spleen, Mouse brain, Mouse testis
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

Northern blot(Homo sapiens)

WB(Homo sapiens)

IF(Homo sapiens)

ABclonal:Western blot - SMARCA5/SNF2H Rabbit pAb (A2000) }

Western blot - SMARCA5/SNF2H Rabbit pAb (A2000)

Western blot analysis of extracts of various cell lines, using SMARCA5/SNF2H antibody (A2000) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.
ABclonal:Immunofluorescence - SMARCA5/SNF2H Rabbit pAb (A2000) }

Immunofluorescence - SMARCA5/SNF2H Rabbit pAb (A2000)

Immunofluorescence analysis of HeLa cells using SMARCA5/SNF2H antibody (A2000). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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