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SLC5A1 Rabbit pAb (A3126)

Publication (1) Datasheet

ABclonal:Western blot - SLC5A1 Rabbit pAb (A3126)
ABclonal:Immunohistochemistry - SLC5A1 Rabbit pAb (A3126)
ABclonal:Immunohistochemistry - SLC5A1 Rabbit pAb (A3126)
ABclonal:Immunohistochemistry - SLC5A1 Rabbit pAb (A3126)
ABclonal:Immunofluorescence - SLC5A1 Rabbit pAb (A3126)
ABclonal:Immunofluorescence - SLC5A1 Rabbit pAb (A3126)

Overview

Product nameSLC5A1 Rabbit pAb
Catalog No.A3126
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide of human SLC5A1
SequenceEmail for sequence
Gene ID6523
Swiss protP13866
SynonymsSLC5A1; D22S675; NAGT; SGLT1
Calculated MW60kDa/73kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, U-937, Mouse kidney, Mouse brain
Cellular locationMembrane, Multi-pass membrane protein
Customer validation

WB(Mus musculus,Sus scrofa)

ABclonal:Western blot - SLC5A1 Rabbit pAb (A3126) }

Western blot - SLC5A1 Rabbit pAb (A3126)

Western blot analysis of extracts of various cell lines, using SLC5A1 antibody (A3126) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.
ABclonal:Immunohistochemistry - SLC5A1 Rabbit pAb (A3126) }

Immunohistochemistry - SLC5A1 Rabbit pAb (A3126)

Immunohistochemistry of paraffin-embedded rat kidney using SLC5A1 antibody (A3126) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SLC5A1 Rabbit pAb (A3126) }

Immunohistochemistry - SLC5A1 Rabbit pAb (A3126)

Immunohistochemistry of paraffin-embedded human gastric cancer using SLC5A1 antibody (A3126) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SLC5A1 Rabbit pAb (A3126) }

Immunohistochemistry - SLC5A1 Rabbit pAb (A3126)

Immunohistochemistry of paraffin-embedded mouse kidney using SLC5A1 antibody (A3126) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - SLC5A1 Rabbit pAb (A3126) }

Immunofluorescence - SLC5A1 Rabbit pAb (A3126)

Immunofluorescence analysis of rat kidney using SLC5A1 Polyclonal Antibody (A3126) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SLC5A1 Rabbit pAb (A3126) }

Immunofluorescence - SLC5A1 Rabbit pAb (A3126)

Immunofluorescence analysis of mouse kidney using SLC5A1 Polyclonal Antibody (A3126) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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