Tag and Loading Control Antibodies
Nucleic Acid Modifying Enzymes
Ubiquitination related reagent
Cytokines & Cytokine Receptors
Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using SKP2 antibody (A0842) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
|Product name||SKP2 Rabbit pAb|
|Purification method||Affinity purification|
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human SKP2 (NP_005974.2).|
|Synonyms||SKP2; FBL1; FBXL1; FLB1; p45|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
|Application key||Western blotting|
|Positive samples||HeLa, K-562, HCT116|
|Cellular location||Cytoplasm, Nucleus|
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Based on existing publications, the following genes are closely related to research on SKP2. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to SKP2. (Distance between topics and target gene indicate popularity.) SKP2
* Data provided by citexs.com, for reference only.
Publishing research using A0842? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.