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SKIV2L2 Polyclonal Antibody (A5576)

Publication (1) Datasheet

ABclonal:Western blot - SKIV2L2 Polyclonal Antibody (A5576)
ABclonal:Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576)
ABclonal:Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576)
ABclonal:Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576)

Overview

Product nameSKIV2L2 Polyclonal Antibody
Catalog No.A5576
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-304 of human SKIV2L2 (NP_056175.3).
SequenceMADAFGDELFSVFEGDSTTAAGTKKDKEKDKGKWKGPPGSADKAGKRFDGKLQSESTNNGKNKRDVDFEGTDEPIFGKKPRIEESITEDLSLADLMPRVKVQSVETVEGCTHEVALPAEEDYLPLKPRVGKAAKEYPFILDAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPC
Gene ID23517
Swiss protP42285
SynonymsSKIV2L2; Dob1; KIAA0052; Mtr4; fSAP118; Ski2 like RNA helicase 2
Calculated MW117kDa
Observed MW123kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, 22RV1, 293T, U-87MG, Mouse testis, Mouse spleen
Cellular locationNucleus, nucleolus, nucleoplasm
Customer validation

IF(Human)

ABclonal:Western blot - SKIV2L2 Polyclonal Antibody (A5576) }

Western blot - SKIV2L2 Polyclonal Antibody (A5576)

Western blot analysis of extracts of various cell lines, using SKIV2L2 antibody (A5576) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576) }

Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576)

Immunohistochemistry of paraffin-embedded human colon using SKIV2L2 antibody (A5576) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576) }

Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576)

Immunohistochemistry of paraffin-embedded human lung cancer using SKIV2L2 antibody (A5576) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576) }

Immunohistochemistry - SKIV2L2 Polyclonal Antibody (A5576)

Immunohistochemistry of paraffin-embedded human esophagus using SKIV2L2 antibody (A5576) at dilution of 1:100 (40x lens).

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