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SIRT1 Rabbit pAb (A0230)

Review (1)Publications (7) Datasheet

ABclonal:Western blot - SIRT1 Rabbit pAb (A0230)

Western blot analysis of extracts of various cell lines, using SIRT1 antibody (A0230) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - SIRT1 Rabbit pAb (A0230)

Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (A0230) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for SIRT1 Rabbit pAb(A0230)

Overview

Product nameSIRT1 Rabbit pAb
Catalog No.A0230
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide of human SIRT1
SequenceEmail for sequence
Gene ID23411
Swiss protQ96EB6
SynonymsSIR2; SIR2L1; SIR2alpha; SIRT1; sirtuin 1
Calculated MW61kDa/81kDa
Observed MW120kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse testis, Rat testis
Cellular locationCytoplasm, Mitochondrion, Nucleus, Nucleus, PML body
Customer validation

(HeLa)

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - SIRT1 Rabbit pAb (A0230) }

Western blot - SIRT1 Rabbit pAb (A0230)

Western blot analysis of extracts of various cell lines, using SIRT1 antibody (A0230) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - SIRT1 Rabbit pAb (A0230) }

Immunofluorescence - SIRT1 Rabbit pAb (A0230)

Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (A0230) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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