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SENP1 Rabbit pAb (A13086)

Datasheet

ABclonal:Western blot - SENP1 Rabbit pAb (A13086)
ABclonal:Immunohistochemistry - SENP1 Rabbit pAb (A13086)
ABclonal:Immunohistochemistry - SENP1 Rabbit pAb (A13086)
ABclonal:Immunohistochemistry - SENP1 Rabbit pAb (A13086)

Overview

Product nameSENP1 Rabbit pAb
Catalog No.A13086
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SENP1 (NP_001254523.1).
SequenceSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIEEQKALALQLQNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDSEDEF
Gene ID29843
Swiss protQ9P0U3
SynonymsSENP1; SuPr-2
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesBxPC-3, HT-29, HepG2, HeLa, Mouse testis, Mouse thymus, Rat testis
Cellular locationCytoplasm, Nucleus
    ABclonal:Western blot - SENP1 Rabbit pAb (A13086) }

    Western blot - SENP1 Rabbit pAb (A13086)

    Western blot analysis of extracts of various cell lines, using SENP1 antibody (A13086) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - SENP1 Rabbit pAb (A13086) }

    Immunohistochemistry - SENP1 Rabbit pAb (A13086)

    Immunohistochemistry of paraffin-embedded Mouse testis using SENP1 Rabbit pAb (A13086) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - SENP1 Rabbit pAb (A13086) }

    Immunohistochemistry - SENP1 Rabbit pAb (A13086)

    Immunohistochemistry of paraffin-embedded Rat testis using SENP1 Rabbit pAb (A13086) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - SENP1 Rabbit pAb (A13086) }

    Immunohistochemistry - SENP1 Rabbit pAb (A13086)

    Immunohistochemistry of paraffin-embedded Human liver cancer using SENP1 Rabbit pAb (A13086) at dilution of 1:100 (40x lens).

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