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SELP Rabbit pAb (A12512)

Datasheet

ABclonal:Western blot - SELP Polyclonal Antibody (A12512)

Overview

Product nameSELP Rabbit pAb
Catalog No.A12512
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 220-319 of human SELP (NP_002996.2).
SequenceFSFNSQCSFHCTDGYQVNGPSKLECLASGIWTNKPPQCLAAQCPPLKIPERGNMTCLHSAKAFQHQSSCSFSCEEGFALVGPEVVQCTASGVWTAPAPVC
Gene ID6403
Swiss protP16109
SynonymsSELP; CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
Calculated MW90kDa
Observed MW100kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse kidney, Rat brain
Cellular locationMembrane, Single-pass type I membrane protein
ABclonal:Western blot - SELP Polyclonal Antibody (A12512) }

Western blot - SELP Polyclonal Antibody (A12512)

Western blot analysis of extracts of various cell lines, using SELP antibody (A12512) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

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