Products > Catalog Antibodies > Polyclonal Antibodies

SELE Rabbit pAb (A2191)

Review (1) Datasheet

ABclonal:Western blot - SELE Polyclonal Antibody (A2191)
ABclonal:Immunohistochemistry - SELE Polyclonal Antibody (A2191)
ABclonal:Immunohistochemistry - SELE Polyclonal Antibody (A2191)
ABclonal:Immunohistochemistry - SELE Polyclonal Antibody (A2191)
ABclonal:Immunofluorescence - SELE Polyclonal Antibody (A2191)
Review
ABclonal: review for SELE Rabbit pAb(A2191)

Overview

Product nameSELE Rabbit pAb
Catalog No.A2191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human SELE (NP_000441.2).
SequenceWSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEW
Gene ID6401
Swiss protP16581
SynonymsSELE; CD62E; ELAM; ELAM1; ESEL; LECAM2
Calculated MW66kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, HepG2, HeLa, Raji, Mouse liver
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

ABclonal:Western blot - SELE Polyclonal Antibody (A2191) }

Western blot - SELE Polyclonal Antibody (A2191)

Western blot analysis of extracts of various cell lines, using SELE antibody (A2191) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - SELE Polyclonal Antibody (A2191) }

Immunohistochemistry - SELE Polyclonal Antibody (A2191)

Immunohistochemistry of paraffin-embedded human breast using SELE Antibody (A2191) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SELE Polyclonal Antibody (A2191) }

Immunohistochemistry - SELE Polyclonal Antibody (A2191)

Immunohistochemistry of paraffin-embedded human uterus using SELE Antibody (A2191) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SELE Polyclonal Antibody (A2191) }

Immunohistochemistry - SELE Polyclonal Antibody (A2191)

Immunohistochemistry of paraffin-embedded human uterine cancer using SELE Antibody (A2191) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - SELE Polyclonal Antibody (A2191) }

Immunofluorescence - SELE Polyclonal Antibody (A2191)

Immunofluorescence analysis of MCF-7 cells using SELE antibody (A2191). Blue: DAPI for nuclear staining.

Submit an review for A2191

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A2191 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order