Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SEL1L Rabbit pAb (A12073)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SEL1L Rabbit pAb (A12073)

Western blot analysis of various lysates, using SEL1L Rabbit pAb (A12073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - SEL1L Rabbit pAb (A12073)

Immunofluorescence analysis of C6 cells using SEL1L antibody (A12073) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SEL1L Rabbit pAb (A12073)

Immunofluorescence analysis of L929 cells using SEL1L antibody (A12073) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Overview

Product name SEL1L Rabbit pAb
Catalog No. A12073
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human SEL1L (NP_005056.3).
Sequence SSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTG
Gene ID 6400
Swiss prot Q9UBV2
Synonyms Hrd3; SEL1L1; PRO1063; SEL1-LIKE; SEL1L
Calculated MW 89kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples U-138MG, Mouse pancreas
Cellular location Endoplasmic reticulum membrane, Single-pass type I membrane protein
Customer validation

IP (Mus musculus)

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

SEL1L Rabbit pAb images

ABclonal:Western blot - SEL1L Rabbit pAb (A12073)}

Western blot - SEL1L Rabbit pAb (A12073)

Western blot analysis of various lysates, using SEL1L Rabbit pAb (A12073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - SEL1L Rabbit pAb (A12073)}

Immunofluorescence - SEL1L Rabbit pAb (A12073)

Immunofluorescence analysis of C6 cells using SEL1L antibody (A12073) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SEL1L Rabbit pAb (A12073)}

Immunofluorescence - SEL1L Rabbit pAb (A12073)

Immunofluorescence analysis of L929 cells using SEL1L antibody (A12073) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SEL1L. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SEL1L. (Distance between topics and target gene indicate popularity.) SEL1L

* Data provided by citexs.com, for reference only.

Publishing research using A12073? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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