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SEC23A Polyclonal Antibody (A12101)

Datasheet

ABclonal:Western blot - SEC23A Polyclonal Antibody (A12101)

Overview

Product nameSEC23A Polyclonal Antibody
Catalog No.A12101
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SEC23A (NP_006355.2).
SequenceMTTYLEFIQQNEERDGVRFSWNVWPSSRLEATRMVVPVAALFTPLKERPDLPPIQYEPVLCSRTTCRAVLNPLCQVDYRAKLWACNFCYQRNQFPPSYAGISELNQPAELLPQFSSIEYVVLRGPQMPLIFLYVVDTCMEDEDLQALKES
Gene ID10484
Swiss protQ15436
SynonymsSEC23A; CLSD; protein transport protein Sec23A
Calculated MW63kDa/86kDa
Observed MW86kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesB cells, LO2, HeLa, SGC-7901, HT-29, Mouse brain, Mouse liver, Rat brain
Cellular locationGolgi apparatus membrane, Peripheral membrane protein, Smooth endoplasmic reticulum membrane
ABclonal:Western blot - SEC23A Polyclonal Antibody (A12101) }

Western blot - SEC23A Polyclonal Antibody (A12101)

Western blot analysis of extracts of various cell lines, using SEC23A antibody (A12101) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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