Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:SARS-CoV-2

ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Western blot analysis of 293T, using SARS-CoV-2Nucleoprotein antibody (A21042) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.

ABclonal:Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Immunofluorescence analysis of 293T-N and 293T using SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name SARS-CoV-2 Nucleoprotein Rabbit mAb
Catalog No. A21042
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC51499

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Sequence MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Gene ID 43740575
Swiss prot P0DTC9
Synonyms N; Nucleoprotein; NC; SARS-CoV-2 Nucleoprotein
Calculated MW 46kDa
Observed MW 50kDa

Applications

Reactivity SARS-CoV-2
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein
Cellular location Virion, Host cytoplasm, Secreted, Host extracellular space;
Customer validation

IHC (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

SARS-CoV-2 Nucleoprotein Rabbit mAb images

ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)}

Western blot - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Western blot analysis of 293T, using SARS-CoV-2Nucleoprotein antibody (A21042) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.
ABclonal:Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)}

Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Immunofluorescence analysis of 293T-N and 293T using SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Nucleoprotein. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Nucleoprotein. (Distance between topics and target gene indicate popularity.) Nucleoprotein

* Data provided by citexs.com, for reference only.

Publishing research using A21042? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Proteins (5)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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