Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SARS-CoV-2 N Protein Rabbit pAb (A20607)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:SARS-CoV-2

ABclonal:Western blot - SARS-CoV-2 N Protein Rabbit pAb (A20607)

Western blot analysis of extracts of Recombinant SARS-CoV Nucleocapsid Protein, using SARS-CoV-2 N Protein Rabbit pAb (A20607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name SARS-CoV-2 N Protein Rabbit pAb
Catalog No. A20607
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of coronavirus N Protein (NP_828858.1).
Sequence MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDG
Gene ID 1489678
Swiss prot P59595
Synonyms
Calculated MW 46kDa
Observed MW 55kDa

Applications

Reactivity SARS-CoV-2
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

SARS-CoV-2 N Protein Rabbit pAb images

ABclonal:Western blot - SARS-CoV-2 N Protein Rabbit pAb (A20607)}

Western blot - SARS-CoV-2 N Protein Rabbit pAb (A20607)

Western blot analysis of extracts of Recombinant SARS-CoV Nucleocapsid Protein, using SARS-CoV-2 N Protein Rabbit pAb (A20607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on Nucleoprotein. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Nucleoprotein. (Distance between topics and target gene indicate popularity.) Nucleoprotein

* Data provided by citexs.com, for reference only.

Publishing research using A20607? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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