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S1PR1 Rabbit pAb (A12935)

Datasheet

ABclonal:Western blot - S1PR1 Polyclonal Antibody (A12935)

Overview

Product nameS1PR1 Rabbit pAb
Catalog No.A12935
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 303-382 of human S1PR1 (NP_001391.2).
SequenceNSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSHPQKDEGDNPETIMSSGNVNSSS
Gene ID1901
Swiss protP21453
SynonymsS1PR1; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; S1P1
Calculated MW42kDa
Observed MW43kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesNIH/3T3, SH-SY5Y, Mouse kidney
Cellular locationCell membrane, Endosome, Membrane raft, Multi-pass membrane protein
ABclonal:Western blot - S1PR1 Polyclonal Antibody (A12935) }

Western blot - S1PR1 Polyclonal Antibody (A12935)

Western blot analysis of extracts of various cell lines, using S1PR1 antibody (A12935) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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