Product Type > Antibodies > Secondary Antibodies

Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Publications (15) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution.
Blue: DAPI for nuclear staining.

Overview

Product nameRhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)
Catalog No.AS040
Host speciesGoat
Purification methodAffinity purification
IsotypeTRITC conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Application keyImmunohistochemistry    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Rattus norvegicus, Homo sapiens, Oryctolagus cuniculus, Leporidae, chicken eggs, Mus musculus, Saccharomyces cerevisiae)

WB(Mus musculus)

Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) images

ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)}

Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution.
Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)}

Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution.
Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Goat Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS040? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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