Products > Catalog Antibodies > Secondary Antibodies

Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Publications (11) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution.
Blue: DAPI for nuclear staining.

Overview

Product nameRhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)
Catalog No.AS040
Host speciesGoat
Purification methodAffinity purification
IsotypeTRITC conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionIHC 1:50 - 1:200
ICC 1:50 - 1:200
IF 1:50 - 1:200
FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: 50% glycerol, pH7.3.
Application keyImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

(Mus musculus)

(Mus musculus)

IF(Rattus norvegicus, Homo sapiens, Oryctolagus cuniculus, Leporidae, chicken eggs, Mus musculus)

    ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)}

    Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

    Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution.
    Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)}

    Immunofluorescence - Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040)

    Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution.
    Blue: DAPI for nuclear staining.

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