Product name | Recombinant Human ACE-2 Protein |
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Catalog No. | RP01266 |
Description | Active Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6×His,Avi tag at the C-terminus. |
Bio-Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 at 2 μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD with a linear range of 0.03-4.9 ng/mL. |
Purity | > 95% by SDS-PAGE. |
Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Calculated MW | 86.2kDa |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term.After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.