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Recombinant SARS-CoV-2 Spike S1 Protein with His tag (RP01262)

Datasheet

ABclonal:Recombinant SARS-CoV-2 Spike S1 Protein with His tag

Overview

Product nameRecombinant SARS-CoV-2 Spike S1 Protein with His tag
Catalog No.RP01262
DescriptionRecombinant SARS-CoV-2(2019-nCoV) Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of SARS-COV-2(2019-nCoV) Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1 at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.|Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay (Nicoya OpenSPR).
Purity>90% by SDS-PAGE;> 95% by HPLC
Endotoxin< 1.0 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesSARS-CoV-2
Calculated MW75.8kDa
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
SynonymS1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein
Expressed HostHEK293 cells
TagC-6×His
Gene ID43740568
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
or This product is stable at ≤ -70°C for up to 6 months from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
ReconstitutionFor lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

* For research use only. Not for therapeutic or diagnostic purposes.

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