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Recombinant SARS-CoV-2 Spike S1 Protein (RP01261)

Publication (1) Datasheet

ABclonal:Recombinant SARS-CoV-2 Spike S1 Protein


Product nameRecombinant SARS-CoV-2 Spike S1 Protein
Catalog No.RP01261
DescriptionRecombinant SARS-CoV-2(2019-nCoV) Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln14-Arg683) of SARS-COV-2(2019-nCoV) Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag and Avi at the C-terminus.
Bio-Activity1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL. 2.Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay (Nicoya OpenSPR).
Purity>95% by SDS-PAGE;> 95% by HPLC
Endotoxin< 1.0 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Calculated MW77.7kDa
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
SynonymS1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein
Expressed HostHEK293 cells
Gene ID43740568
StorageStore the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
or This product is stable at ≤ -70°C for up to 1 year from the date of receipt.
For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
ReconstitutionFor lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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