Product Type > Antibodies > Tag and Loading Control Antibodies

Rabbit anti V5-Tag pAb (AE022)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - Rabbit anti V5-Tag pAb (AE022)

Western blot analysis of over-expressed V5-tagged protein in 293T cell using V5-tag antibody (AE022) at different dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.

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Overview

Product name Rabbit anti V5-Tag pAb
Catalog No. AE022
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Immunogen information

Immunogen A synthetic peptide corresponding to V5 tag.
Sequence GKPIPNPLLGLDST
Gene ID
Swiss prot
Synonyms V5; V5 tag; V5-tag
Calculated MW
Observed MW Refer to Figures

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples
Cellular location

Rabbit anti V5-Tag pAb images

ABclonal:Western blot - Rabbit anti V5-Tag pAb (AE022)}

Western blot - Rabbit anti V5-Tag pAb (AE022)

Western blot analysis of over-expressed V5-tagged protein in 293T cell using V5-tag antibody (AE022) at different dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.

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Related Targets

Based on existing publications, the following genes are closely related to research on V5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to V5. (Distance between topics and target gene indicate popularity.) V5

* Data provided by citexs.com, for reference only.

Publishing research using AE022? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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