Product Type > Antibodies > Tag and Loading Control Antibodies

DDDDK-Tag Rabbit mAb (AE063)

Publications (34) Datasheet SDS COA

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:DDDDK-Tag Rabbit mAb
ABclonal:Western blot - DDDDK-Tag Rabbit mAb (AE063)

Western blot analysis of 293T, using DDDDK-Tag antibody (AE063) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody (AE063). Green: GFP expression. Blue: DAPI for nuclear staining. 

ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using DDDDK-Tag Rabbit mAb (AE063) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063), Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation analysis of 600ug extracts of 293T cells using 3ug DDDDK-Tag antibody (AE063). Western blot was performed from the immunoprecipitate using DDDDK-Tag (AE063) at a dilution of 1:1000.

Overview

Product nameDDDDK-Tag Rabbit mAb
Catalog No.AE063
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5111-02

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Immunogen information

ImmunogenA synthetic peptide corresponding to DDDDK tag.
SequenceDYKDDDDK
Gene ID
Swiss prot
SynonymsDDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MWRefer to Figures

Applications

Reactivity
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:2000 - 1:6000
  • IHC 1:50 - 1:500
  • IF/ICC 1:50 - 1:200
  • IP 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T
Cellular location
Customer validation

WB(Oreochroms mossambcus, Mus musculus, Homo sapiens, Other, Arabidopsis thaliana, Human, Bombyx mori Linnaeus)

IHC(Mus musculus)

IF(Mus musculus)

ChIP(Arabidopsis thaliana)

IP(Homo sapiens)

WB(Homo sapiens)

IP(Homo sapiens,Mus musculus, Other, Arabidopsis thaliana)

IHC(Mus musculus, Homo sapiens)

IF(Mus musculus)

Co-IP(Glycine max L)

RIP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

DDDDK-Tag Rabbit mAb images

ABclonal:Western blot - DDDDK-Tag Rabbit mAb (AE063)}

Western blot - DDDDK-Tag Rabbit mAb (AE063)

Western blot analysis of 293T, using DDDDK-Tag antibody (AE063) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)}

Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody (AE063). Green: GFP expression. Blue: DAPI for nuclear staining. 
ABclonal:Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)}

Immunofluorescence - DDDDK-Tag Rabbit mAb (AE063)

Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using DDDDK-Tag Rabbit mAb (AE063) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)}

Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063), Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
ABclonal:Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)}

Immunoprecipitation - DDDDK-Tag Rabbit mAb (AE063)

Immunoprecipitation analysis of 600ug extracts of 293T cells using 3ug DDDDK-Tag antibody (AE063). Western blot was performed from the immunoprecipitate using DDDDK-Tag (AE063) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on DDDDK. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to DDDDK. (Distance between topics and target gene indicate popularity.) DDDDK

* Data provided by citexs.com, for reference only.

Publishing research using AE063? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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