Publications (34) Datasheet SDS COA
Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:
Western blot analysis of 293T, using DDDDK-Tag antibody (AE063) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody (AE063). Green: GFP expression. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using DDDDK-Tag Rabbit mAb (AE063) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063), Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
| Product name | DDDDK-Tag Rabbit mAb |
|---|---|
| Catalog No. | AE063 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC5111-02 |
| Immunogen | A synthetic peptide corresponding to DDDDK tag. |
|---|---|
| Sequence | DYKDDDDK |
| Gene ID | |
| Swiss prot | |
| Synonyms | DDDDK; DDDDK tag; DDDDK-tag |
| Calculated MW | |
| Observed MW | Refer to Figures |
| Reactivity | |
|---|---|
| Tested applications | WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunoprecipitation |
| Positive samples | 293T |
| Cellular location | |
| Customer validation | WB(Oreochroms mossambcus, Mus musculus, Homo sapiens, Other, Arabidopsis thaliana, Human, Bombyx mori Linnaeus) IHC(Mus musculus) IF(Mus musculus) ChIP(Arabidopsis thaliana) IP(Homo sapiens) WB(Homo sapiens) IP(Homo sapiens,Mus musculus, Other, Arabidopsis thaliana) IHC(Mus musculus, Homo sapiens) IF(Mus musculus) Co-IP(Glycine max L) RIP(Homo sapiens) |
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Based on existing publications, the following genes are closely related to research on DDDDK. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to DDDDK. (Distance between topics and target gene indicate popularity.) DDDDK
* Data provided by citexs.com, for reference only.
Publishing research using AE063? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
