Products > Catalog Antibodies > Secondary Antibodies

Rabbit Anti-Chicken IgY (H+L) (AS082)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:

ABclonal:Western blot - Rabbit Anti-Chicken IgY (H+L) (AS082)

Western blot analysis of extracts of Chicken IgY, using Rabbit Anti-Chicken IgY (H+L) antibody (AS082) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameRabbit Anti-Chicken IgY (H+L)
Catalog No.AS082
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenChicken IgY
SequenceEmail for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW25KDa/67-70KDa
Reactivity
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesRabbit Anti-Chicken IgY
Cellular location
    ABclonal:Western blot - Rabbit Anti-Chicken IgY (H+L) (AS082) }

    Western blot - Rabbit Anti-Chicken IgY (H+L) (AS082)

    Western blot analysis of extracts of Chicken IgY, using Rabbit Anti-Chicken IgY (H+L) antibody (AS082) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

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