Product Type > Antibodies > Secondary Antibodies

Rabbit Anti-Chicken IgY Fab (AS088)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - Rabbit Anti-Chicken IgY Fab (AS088)

Western blot analysis of Chicken IgY Fab, using Chicken IgY Fab antibody (AS088) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Rabbit Anti-Chicken IgY Fab
Catalog No. AS088
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC56601

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Chicken IgY
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW 25kDa

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:20000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Chicken IgY Fab
Cellular location

Documents

Certificate of Compliance

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Lot number

Rabbit Anti-Chicken IgY Fab images

ABclonal:Western blot - Rabbit Anti-Chicken IgY Fab (AS088)}

Western blot - Rabbit Anti-Chicken IgY Fab (AS088)

Western blot analysis of Chicken IgY Fab, using Chicken IgY Fab antibody (AS088) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on Rabbit Anti-Chicken. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Rabbit Anti-Chicken. (Distance between topics and target gene indicate popularity.) Rabbit Anti-Chicken

* Data provided by citexs.com, for reference only.

Publishing research using AS088? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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