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RUNX2 Polyclonal Antibody (A2851)

Reviews (2)Publications (8) Datasheet

ABclonal:Western blot - RUNX2 Polyclonal Antibody (A2851)
Review
ABclonal: review for RUNX2 Polyclonal Antibody(A2851)
Review
ABclonal: review for RUNX2 Polyclonal Antibody(A2851)

Overview

Product nameRUNX2 Polyclonal Antibody
Catalog No.A2851
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 242-521 of human RUNX2 (NP_001019801.3).
SequenceFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSTLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSGSYQFPMVPGGDRSPSRMLPPCTTTSNGSTLLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY
Gene ID860
Swiss protQ13950
SynonymsRUNX2; AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2aA; runt-related transcription factor 2
Calculated MW54kDa/55kDa/56kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-251MG, K-562, Mouse thymus, Mouse spleen
Cellular locationNucleus
Customer validation

(, )

WB(Human, Mouse, Human, Mouse)

IHC(Mouse)

IHC(Human,Mouse)

ABclonal:Western blot - RUNX2 Polyclonal Antibody (A2851) }

Western blot - RUNX2 Polyclonal Antibody (A2851)

Western blot analysis of extracts of various cell lines, using RUNX2 antibody (A2851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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