Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

RPS17 Rabbit pAb (A16426)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS17 Rabbit pAb (A16426)

Western blot analysis of various lysates using RPS17 Rabbit pAb (A16426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - RPS17 Rabbit pAb (A16426)

Immunofluorescence analysis of L929 cells using RPS17 Rabbit pAb (A16426) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS17 Rabbit pAb (A16426)

Immunofluorescence analysis of U-2 OS cells using RPS17 Rabbit pAb (A16426) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS17 Rabbit pAb (A16426)

Immunofluorescence analysis of U-2 OS cells using RPS17 Rabbit pAb (A16426) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name RPS17 Rabbit pAb
Catalog No. A16426
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS17 (NP_001012.1).
Sequence MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDP
Gene ID 6218
Swiss prot P08708
Synonyms S17; DBA4; eS17; RPS17L; RPS17L1; RPS17L2; RPS17
Calculated MW 16kDa
Observed MW 20kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T, A-549, DU145, BxPC-3, NIH/3T3, mouse brain
Cellular location cytoplasm, cytosol, cytosolic ribosome, focal adhesion, nucleoplasm

Documents

Certificate of Compliance

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RPS17 Rabbit pAb images

ABclonal:Western blot - RPS17 Rabbit pAb (A16426)}

Western blot - RPS17 Rabbit pAb (A16426)

Western blot analysis of various lysates using RPS17 Rabbit pAb (A16426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - RPS17 Rabbit pAb (A16426)}

Immunofluorescence - RPS17 Rabbit pAb (A16426)

Immunofluorescence analysis of L929 cells using RPS17 Rabbit pAb (A16426) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPS17 Rabbit pAb (A16426)}

Immunofluorescence - RPS17 Rabbit pAb (A16426)

Immunofluorescence analysis of U-2 OS cells using RPS17 Rabbit pAb (A16426) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPS17 Rabbit pAb (A16426)}

Immunofluorescence - RPS17 Rabbit pAb (A16426)

Immunofluorescence analysis of U-2 OS cells using RPS17 Rabbit pAb (A16426) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RPS17. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RPS17. (Distance between topics and target gene indicate popularity.) RPS17

* Data provided by citexs.com, for reference only.

Publishing research using A16426? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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