Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

RPA2 Rabbit pAb (A2189)

Reviews (2) Publications (9) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - RPA2 Rabbit pAb (A2189)

Western blot analysis of various lysates using RPA2 Rabbit pAb (A2189) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - RPA2 Rabbit pAb (A2189)

Immunofluorescence analysis of C6 cells using RPA2 Rabbit pAb (A2189) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - RPA2 Rabbit pAb (A2189)

Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug RPA2 Rabbit pAb antibody (A2189 1:100). Western blot was performed from the immunoprecipitate using RPA2 Rabbit pAb antibody (A2189) at a dilition of 1:1000.

Review
ABclonal: review for RPA2 Rabbit pAb(A2189)
Review
ABclonal: review for RPA2 Rabbit pAb(A2189)

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Overview

Product name RPA2 Rabbit pAb
Catalog No. A2189
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1).
Sequence IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE
Gene ID 6118
Swiss prot P15927
Synonyms REPA2; RPA32; RP-A p32; RP-A p34; RPA2
Calculated MW 29kDa
Observed MW 32kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, 293T+UV, NIH/3T3, C6
Cellular location Nucleus, PML body
Customer validation

WB (Mouse liver, Mouse adipose-derived stem cellsHomo sapiens, Homo sapiens, Triticum aestivum L., Mus musculus)

IF (Mus musculus)

IHC (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

RPA2 Rabbit pAb images

ABclonal:Western blot - RPA2 Rabbit pAb (A2189)}

Western blot - RPA2 Rabbit pAb (A2189)

Western blot analysis of various lysates using RPA2 Rabbit pAb (A2189) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - RPA2 Rabbit pAb (A2189)}

Immunofluorescence - RPA2 Rabbit pAb (A2189)

Immunofluorescence analysis of C6 cells using RPA2 Rabbit pAb (A2189) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - RPA2 Rabbit pAb (A2189)}

Immunoprecipitation - RPA2 Rabbit pAb (A2189)

Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug RPA2 Rabbit pAb antibody (A2189 1:100). Western blot was performed from the immunoprecipitate using RPA2 Rabbit pAb antibody (A2189) at a dilition of 1:1000.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on RPA2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RPA2. (Distance between topics and target gene indicate popularity.) RPA2

* Data provided by citexs.com, for reference only.

Publishing research using A2189? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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