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ROCK2 Rabbit mAb (A2395)

Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - ROCK2 Rabbit mAb (A2395)

Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (A2395) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ROCK2 Rabbit mAb (A2395)

Immunohistochemistry of paraffin-embedded human esophageal using ROCK2 Rabbit mAb (A2395) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameROCK2 Rabbit mAb
Catalog No.A2395
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1289-1388 of human ROCK2 (O75116).
SequencePALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS
Gene ID9475
Swiss protO75116
SynonymsROCK-II
Calculated MW161kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, U-87MG, Mouse lung, Mouse brain, Rat brain
Cellular locationCell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - ROCK2 Rabbit mAb (A2395)}

Western blot - ROCK2 Rabbit mAb (A2395)

Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (A2395) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ROCK2 Rabbit mAb (A2395)}

Immunohistochemistry - ROCK2 Rabbit mAb (A2395)

Immunohistochemistry of paraffin-embedded human esophageal using ROCK2 Rabbit mAb (A2395) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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