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RNF20 Rabbit pAb (A17309)

Publication (1) Datasheet

ABclonal:Western blot - RNF20 Polyclonal Antibody (A17309)

Western blot analysis of extracts of various cell lines, using RNF20 antibody (A17309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameRNF20 Rabbit pAb
Catalog No.A17309
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF20 (NP_062538.5).
SequenceMSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETA
Gene ID56254
Swiss protQ5VTR2
SynonymsRNF20; BRE1; BRE1A; hBRE1
Calculated MW113kDa
Observed MW114kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-87MG, 22Rv1, 293T, OVCAR3, HeLa, mouse kidney, mouse liver, rat kidney, rat liver
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - RNF20 Polyclonal Antibody (A17309) }

Western blot - RNF20 Polyclonal Antibody (A17309)

Western blot analysis of extracts of various cell lines, using RNF20 antibody (A17309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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