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RIPK3 Rabbit pAb (A5431)

Review (1)Publications (13) Datasheet

ABclonal:Western blot - RIPK3 Rabbit pAb (A5431)
ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431)
ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431)
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)
Review
ABclonal: review for RIPK3 Rabbit pAb(A5431)

Overview

Product nameRIPK3 Rabbit pAb
Catalog No.A5431
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RIPK3 (NP_006862.2).
SequenceMSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRL
Gene ID11035
Swiss protQ9Y572
SynonymsRIPK3; RIP3
Calculated MW25kDa/27kDa/56kDa
Observed MW57KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29
Cellular locationCell membrane, Cytoplasm, Mitochondrion, cytosol
Customer validation

(human kidney cancer cells)

WB(Homo sapiens, Mus musculus, Gallus gallus, Ctenopharyngodon, Rattus norvegicus, Ctenopharyngodon idellus)

IHC(Mus musculus)

IHC(Gallus gallus)

ABclonal:Western blot - RIPK3 Rabbit pAb (A5431) }

Western blot - RIPK3 Rabbit pAb (A5431)

Western blot analysis of extracts of HT-29 cells, using RIPK3 Rabbit pAb (A5431) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431) }

Immunohistochemistry - RIPK3 Rabbit pAb (A5431)

Immunohistochemistry of paraffin-embedded human liver cancer using RIPK3 antibody (A5431) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431) }

Immunohistochemistry - RIPK3 Rabbit pAb (A5431)

Immunohistochemistry of paraffin-embedded mouse pancreas using RIPK3 antibody (A5431) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431) }

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of C6 cells using RIPK3 antibody (A5431) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431) }

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of L929 cells using RIPK3 antibody (A5431) at dilution of 1:100. Blue: DAPI for nuclear staining.

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