Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

RIPK3 Rabbit pAb (A5431)

Review (1) Publications (44) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - RIPK3 Rabbit pAb (A5431)

Western blot analysis of various lysates, using RIPK3 Rabbit pAb (A5431) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431)

Immunohistochemistry analysis of RIPK3 in paraffin-embedded human liver cancer using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431)

Immunohistochemistry analysis of RIPK3 in paraffin-embedded mouse pancreas using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of C6 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of NIH/3T3 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of PC-12 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of HT-29 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of NIH/3T3 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of PC-12 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for RIPK3 Rabbit pAb(A5431)

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Overview

Product name RIPK3 Rabbit pAb
Catalog No. A5431
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Immunogen information

Immunogen Recombinant Protein corresponding to a sequence within amino acids 21-287 of human RIP3 (NP_006862.2).
Sequence LENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVF
Gene ID 11035
Swiss prot Q9Y572
Synonyms RIP3; RIPK3
Calculated MW 57kDa
Observed MW 57kDa/46-62kDa(M)

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa(Negative control), L929
Cellular location Cell membrane, Cytoplasm, Mitochondrion, cytosol
Customer validation

WB (human kidney cancer cellsHomo sapiens, Gallus gallus, Homo sapiens, Mus musculus, Ctenopharyngodon, Rattus norvegicus, Ctenopharyngodon idellus, Marine Streptomyces sp, Sus scrofa, Ctenopharyngodon idella)

IHC (Mus musculus, Gallus gallus, Rattus norvegicus, Homo sapiens)

ICH (Mus musculus)

IF (Mus musculus, Rattus norvegicus)

ELISA (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

RIPK3 Rabbit pAb images

ABclonal:Western blot - RIPK3 Rabbit pAb (A5431)}

Western blot - RIPK3 Rabbit pAb (A5431)

Western blot analysis of various lysates, using RIPK3 Rabbit pAb (A5431) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431)}

Immunohistochemistry - RIPK3 Rabbit pAb (A5431)

Immunohistochemistry analysis of RIPK3 in paraffin-embedded human liver cancer using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RIPK3 Rabbit pAb (A5431)}

Immunohistochemistry - RIPK3 Rabbit pAb (A5431)

Immunohistochemistry analysis of RIPK3 in paraffin-embedded mouse pancreas using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)}

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of C6 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)}

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of NIH/3T3 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)}

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of PC-12 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)}

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of HT-29 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)}

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of NIH/3T3 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A5431)}

Immunofluorescence - RIPK3 Rabbit pAb (A5431)

Immunofluorescence analysis of PC-12 cells using RIPK3 Rabbit pAb (A5431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on RIPK3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RIPK3. (Distance between topics and target gene indicate popularity.) RIPK3

* Data provided by citexs.com, for reference only.

Publishing research using A5431? Please let us know so that we can cite the reference in this datasheet.

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