Products > Catalog Antibodies > Polyclonal Antibodies

RIPK3 Rabbit pAb (A12996)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using RIPK3 antibody (A12996) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A12996)

Immunofluorescence analysis of Jurkat cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	RIPK3 Rabbit pAb
Catalog No.	A12996
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

Immunogen information

Immunogen	Recombinant protein of mouse RIPK3
Sequence	Email for sequence
Gene ID	56532
Swiss prot	E9PZP3
Synonyms	RIPK3; RIP3
Calculated MW	46kDa
Observed MW	50-57kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IF 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunofluorescence
Positive samples	HT-29, BxPC-3, NIH/3T3, Rat spleen
Cellular location	Cell membrane, Cytoplasm, Mitochondrion, cytosol
Customer validation	WB(Homo sapiens)