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RIPK3 Rabbit pAb (A12996)

Publication (1) Datasheet

ABclonal:Western blot - RIPK3 Rabbit pAb (A12996)
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A12996)
ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A12996)

Overview

Product nameRIPK3 Rabbit pAb
Catalog No.A12996
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
ImmunogenRecombinant protein of mouse RIPK3
SequenceEmail for sequence
Gene ID56532
Swiss protE9PZP3
SynonymsRIPK3; RIP3
Calculated MW46kDa
Observed MW50-57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHT-29, BxPC-3, NIH/3T3, Rat spleen
Cellular locationCell membrane, Cytoplasm, Mitochondrion, cytosol
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - RIPK3 Rabbit pAb (A12996) }

    Western blot - RIPK3 Rabbit pAb (A12996)

    Western blot analysis of extracts of various cell lines, using RIPK3 antibody (A12996) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A12996) }

    Immunofluorescence - RIPK3 Rabbit pAb (A12996)

    Immunofluorescence analysis of Jurkat cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A12996) }

    Immunofluorescence - RIPK3 Rabbit pAb (A12996)

    Immunofluorescence analysis of RAW264.7 cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Blue: DAPI for nuclear staining.

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