Western blot analysis of extracts of various cell lines, using RIPK3 antibody (A12996) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunofluorescence analysis of Jurkat cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | RIPK3 Rabbit pAb |
|---|---|
| Catalog No. | A12996 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant protein of mouse RIPK3 |
|---|---|
| Sequence | Email for sequence |
| Gene ID | 56532 |
| Swiss prot | E9PZP3 |
| Synonyms | RIPK3; RIP3 |
| Calculated MW | 46kDa |
| Observed MW | 50-57kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunofluorescence |
| Positive samples | HT-29, BxPC-3, NIH/3T3, Rat spleen |
| Cellular location | Cell membrane, Cytoplasm, Mitochondrion, cytosol |
| Customer validation | WB(Homo sapiens) |
* For research use only. Not for therapeutic or diagnostic purposes.
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