Products > Catalog Antibodies > Polyclonal Antibodies

RIPK1 Rabbit pAb (A7414)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - RIPK1 Rabbit pAb (A7414)
ABclonal:Western blot - RIPK1 Rabbit pAb (A7414)
ABclonal:Immunohistochemistry - RIPK1 Rabbit pAb (A7414)
ABclonal:Immunohistochemistry - RIPK1 Rabbit pAb (A7414)
ABclonal:Immunohistochemistry - RIPK1 Rabbit pAb (A7414)
Review
ABclonal: review for RIPK1 Rabbit pAb(A7414)

Overview

Product nameRIPK1 Rabbit pAb
Catalog No.A7414
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2).
SequenceNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYS
Gene ID8737
Swiss protQ13546
SynonymsRIP; RIP-1; RIP1; RIPK1
Calculated MW70kDa/75kDa
Observed MW76kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesJurkat, HT-1080, DU145, K-562, NIH/3T3, Mouse liver, C6
Cellular locationCell membrane, Cytoplasm
Customer validation

(human kidney cancer cells)

WB(Gallus gallus)

IHC(Gallus gallus)

ABclonal:Western blot - RIPK1 Rabbit pAb (A7414) }

Western blot - RIPK1 Rabbit pAb (A7414)

Western blot analysis of extracts of various cell lines, using RIPK1 antibody (A7414) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - RIPK1 Rabbit pAb (A7414) }

Western blot - RIPK1 Rabbit pAb (A7414)

Western blot analysis of extracts of various cell lines, using RIPK1 antibody (A7414) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - RIPK1 Rabbit pAb (A7414) }

Immunohistochemistry - RIPK1 Rabbit pAb (A7414)

Immunohistochemistry of paraffin-embedded human tonsil using RIPK1 antibody (A7414) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - RIPK1 Rabbit pAb (A7414) }

Immunohistochemistry - RIPK1 Rabbit pAb (A7414)

Immunohistochemistry of paraffin-embedded rat lung using RIPK1 antibody (A7414) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - RIPK1 Rabbit pAb (A7414) }

Immunohistochemistry - RIPK1 Rabbit pAb (A7414)

Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1 antibody (A7414) at dilution of 1:100 (40x lens).

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