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RIPK1/RIP Rabbit mAb (A19580)

Publications (3) Datasheet

ABclonal:Western blot - RIPK1/RIP Rabbit mAb (A19580)

Western blot analysis of extracts of various cell lines, using RIPK1/RIP antibody (A19580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunoprecipitation - RIPK1/RIP Rabbit mAb (A19580)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RIPK1/RIP antibody (A19580). Western blot was performed from the immunoprecipitate using RIPK1/RIP antibody (A19580) at a dilition of 1:500.

Overview

Product nameRIPK1/RIP Rabbit mAb
Catalog No.A19580
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthesized peptide derived from human RIPK1/RIP.
SequenceEmail for sequence
Gene ID8737
Swiss protQ13546
SynonymsRIP; RIP-1; RIP1; RIPK1
Calculated MW75kDa
Observed MW75KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesRaji, HeLa
Cellular locationCell membrane, Cytoplasm
Customer validation

WB(Homo sapiens,Mus musculus, Ctenopharyngodon)

ABclonal:Western blot - RIPK1/RIP Rabbit mAb (A19580) }

Western blot - RIPK1/RIP Rabbit mAb (A19580)

Western blot analysis of extracts of various cell lines, using RIPK1/RIP antibody (A19580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunoprecipitation - RIPK1/RIP Rabbit mAb (A19580) }

Immunoprecipitation - RIPK1/RIP Rabbit mAb (A19580)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RIPK1/RIP antibody (A19580). Western blot was performed from the immunoprecipitate using RIPK1/RIP antibody (A19580) at a dilition of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

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