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RIPK1 Rabbit mAb (A19580)

Publications (3) Datasheet

ABclonal:Western blot - RIPK1 Rabbit mAb (A19580)
ABclonal:Immunoprecipitation - RIPK1 Rabbit mAb (A19580)

Overview

Product nameRIPK1 Rabbit mAb
Catalog No.A19580
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthesized peptide derived from human RIPK1.
SequenceEmail for sequence
Gene ID8737
Swiss protQ13546
SynonymsRIP; RIP-1; RIP1; RIPK1
Calculated MW75kDa
Observed MW75KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesRaji, HeLa
Cellular locationCell membrane, Cytoplasm
Customer validation

WB(Homo sapiens, Ctenopharyngodon, Mus musculus)

ABclonal:Western blot - RIPK1 Rabbit mAb (A19580) }

Western blot - RIPK1 Rabbit mAb (A19580)

Western blot analysis of extracts of various cell lines, using RIPK1 antibody (A19580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunoprecipitation - RIPK1 Rabbit mAb (A19580) }

Immunoprecipitation - RIPK1 Rabbit mAb (A19580)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RIPK1 antibody (A19580). Western blot was performed from the immunoprecipitate using RIPK1 antibody (A19580) at a dilition of 1:500.

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