Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

RAB4A Rabbit mAb (A11143)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - RAB4A Rabbit mAb (A11143)

Western blot analysis of various lysates using RAB4A Rabbit mAb (A11143).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name RAB4A Rabbit mAb
Catalog No. A11143
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

Immunogen information

Immunogen Recombinant protein of human RAB4A
Sequence Email for sequence
Gene ID 5867
Swiss prot P20338
Synonyms RAB4; HRES1; HRES-1; HRES-1/RAB4; RAB4A
Calculated MW 24kDa
Observed MW 24kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Immunoprecipitation    
Positive samples MCF7, 293T
Cellular location Cytoplasm, Membrane, Peripheral membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

RAB4A Rabbit mAb images

ABclonal:Western blot - RAB4A Rabbit mAb (A11143)}

Western blot - RAB4A Rabbit mAb (A11143)

Western blot analysis of various lysates using RAB4A Rabbit mAb (A11143).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on RAB4A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RAB4A. (Distance between topics and target gene indicate popularity.) RAB4A

* Data provided by citexs.com, for reference only.

Publishing research using A11143? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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