Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Pituitary homeobox 2 (PITX2) Rabbit mAb (A22173)

Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human

ABclonal:Western blot - Pituitary homeobox 2 (PITX2) Rabbit mAb (A22173)

Western blot analysis of HeLa, using Pituitary homeobox 2 (PITX2) antibody (A22173) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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    Overview

    Product namePituitary homeobox 2 (PITX2) Rabbit mAb
    Catalog No.A22173
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC56103

    Background

    This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pituitary homeobox 2 (PITX2) (NP_700475.1).
    SequenceMETNCRKLVSACVQLGVQPAAVECLFSKDSEIKKVEFTDSPESRKEAASSKFFPRQHPGANEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQEL
    Gene ID5308
    Swiss protQ99697
    SynonymsRS; RGS; ARP1; Brx1; IDG2; IGDS; IHG2; PTX2; RIEG; ASGD4; IGDS2; IRID2; Otlx2; RIEG1
    Calculated MW35kDa
    Observed MW37kDa

    Applications

    ReactivityHuman
    Tested applicationsTesting results
    WBHuman
    Recommended dilution
    • WB 1:1000 - 1:5000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    
    Positive samplesHeLa
    Cellular locationNucleus

    Documents

    Certificate of Compliance

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    Lot number

    Research Area

    Pituitary homeobox 2 (PITX2) Rabbit mAb images

    ABclonal:Western blot - Pituitary homeobox 2 (PITX2) Rabbit mAb (A22173)}

    Western blot - Pituitary homeobox 2 (PITX2) Rabbit mAb (A22173)

    Western blot analysis of HeLa, using Pituitary homeobox 2 (PITX2) antibody (A22173) at1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on PITX2. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to PITX2. (Distance between topics and target gene indicate popularity.) PITX2

    * Data provided by citexs.com, for reference only.

    Publishing research using A22173? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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