Western blot analysis of extracts of NIH/3T3 cells, using Phospho-P70S6K1-T389 pAb (AP0564) at 1:2000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse heart using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.
Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.
|Product name||Phospho-p70 S6 kinase-T389 Rabbit pAb|
|Purification method||Affinity purification|
|Immunogen||A phospho specific peptide corresponding to residues surrounding T389 of human P70 S6K|
|Sequence||Email for sequence|
|Synonyms||PS6K; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70(S6K)-alpha; p70-S6K; p70-alpha; P70 S6K; RPS6KB1; p70S6KA|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IHC 1:50 - 1:200
IF 1:50 - 1:200
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry Immunofluorescence|
|Cellular location||Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome|
(MSC cells, unknow)
WB(Homo sapiens, Mus musculus, Rattus norvegicus, Spodoptera frugiperda)
* For research use only. Not for therapeutic or diagnostic purposes.
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