Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Reviews (2)Publications (8) Datasheet

ABclonal:Western blot - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-P70S6K1-T389 pAb (AP0564) at 1:2000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunohistochemistry of paraffin-embedded mouse heart using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of C6 cells using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.

ABclonal:Immunofluorescence - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.

Review
ABclonal: review for Phospho-p70 S6 kinase-T389 Rabbit pAb(AP0564)
Review
ABclonal: review for Phospho-p70 S6 kinase-T389 Rabbit pAb(AP0564)

Overview

Product namePhospho-p70 S6 kinase-T389 Rabbit pAb
Catalog No.AP0564
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
ImmunogenA phospho specific peptide corresponding to residues surrounding T389 of human P70 S6K
SequenceEmail for sequence
Gene ID6198
Swiss protP23443
SynonymsPS6K; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70(S6K)-alpha; p70-S6K; p70-alpha; P70 S6K; RPS6KB1; p70S6KA
Calculated MW51kDa/52kDa/56kDa/59kDa
Observed MW90KDa/100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3
Cellular locationCell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome
Customer validation

(MSC cells, unknow)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Spodoptera frugiperda)

ABclonal:Western blot - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564) }

Western blot - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-P70S6K1-T389 pAb (AP0564) at 1:2000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564) }

Immunohistochemistry - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunohistochemistry of paraffin-embedded mouse heart using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564) }

Immunohistochemistry - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564) }

Immunofluorescence - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of C6 cells using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.
ABclonal:Immunofluorescence - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564) }

Immunofluorescence - Phospho-p70 S6 kinase-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 kinase-T389 antibody (AP0564) at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for AP0564

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about AP0564 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AP0564? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order