Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p53-S37 Rabbit pAb (AP1110)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p53-S37 Rabbit pAb (AP1110)

Western blot analysis of various lysates using Phospho-p53-S37 Rabbit pAb (AP1110) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-p53-S37 Rabbit pAb (AP1110)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S37 Rabbit pAb (AP1110) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Mouse kidney tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Human colon carcinoma tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Rat liver tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Human breast cancer tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

You may also interested in:

Overview

Product name Phospho-p53-S37 Rabbit pAb
Catalog No. AP1110
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen A synthetic phosphorylated peptide around S37 of human p53 (NP_000537.3).
Sequence LPSQA
Gene ID 7157
Swiss prot P04637
Synonyms P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-S37
Calculated MW 44kDa
Observed MW 53kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T, NIH/3T3, C6
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-p53-S37 Rabbit pAb images

ABclonal:Western blot - Phospho-p53-S37 Rabbit pAb (AP1110)}

Western blot - Phospho-p53-S37 Rabbit pAb (AP1110)

Western blot analysis of various lysates using Phospho-p53-S37 Rabbit pAb (AP1110) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-p53-S37 Rabbit pAb (AP1110)}

Western blot - Phospho-p53-S37 Rabbit pAb (AP1110)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S37 Rabbit pAb (AP1110) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)}

Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Mouse kidney tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)}

Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Human colon carcinoma tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)}

Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Rat liver tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)}

Immunohistochemistry - Phospho-p53-S37 Rabbit pAb (AP1110)

Immunohistochemistry analysis of Phospho-p53-S37 in paraffin-embedded Human breast cancer tissue using Phospho-p53-S37 Rabbit pAb (AP1110) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Inquire About This Product

Submit your question about AP1110 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

Publishing research using AP1110? Please let us know so that we can cite the reference in this datasheet.

Antibodies (31)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order