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Phospho-p53-S15 pAb (AP0083)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - Phospho-p53-S15 pAb (AP0083)
ABclonal:Immunofluorescence - Phospho-p53-S15 pAb (AP0083)
ABclonal:Immunofluorescence - Phospho-p53-S15 pAb (AP0083)
ABclonal:Immunoprecipitation - Phospho-p53-S15 pAb (AP0083)
Review
ABclonal: review for Phospho-p53-S15 pAb(AP0083)
Review
ABclonal: review for Phospho-p53-S15 pAb(AP0083)

Overview

Product namePhospho-p53-S15 pAb
Catalog No.AP0083
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
ImmunogenA synthetic phosphorylated peptide around S15 of human p53 (NP_000537.3).
SequencePLSQE
Gene ID7157
Swiss protP04637
SynonymsBCC7; LFS1; P53; TRP53; p53; TP53; BMFS5
Calculated MW23-43kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHT-29, 293
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

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WB(Human)

ABclonal:Western blot - Phospho-p53-S15 pAb (AP0083) }

Western blot - Phospho-p53-S15 pAb (AP0083)

Western blot analysis of extracts of HT-29 and 293 cells, using Phospho-p53-S15 antibody (AP0083) at 1:1000 dilution. HT-29 cells were treated by UV for 15-30 minutes. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.
ABclonal:Immunofluorescence - Phospho-p53-S15 pAb (AP0083) }

Immunofluorescence - Phospho-p53-S15 pAb (AP0083)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-p53-S15 antibody (AP0083). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
ABclonal:Immunofluorescence - Phospho-p53-S15 pAb (AP0083) }

Immunofluorescence - Phospho-p53-S15 pAb (AP0083)

Immunofluorescence analysis of U2OS cells using Phospho-p53-S15 antibody (AP0083). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Phospho-p53-S15 pAb (AP0083) }

Immunoprecipitation - Phospho-p53-S15 pAb (AP0083)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-p53-S15 pAb (AP0083). Western blot was performed from the immunoprecipitate using Phospho-p53-S15 pAb (AP0083) at a dilition of 1:1000. 293T cells were treated by UV at room temperature for 30 minutes after serum-starvation overnight, and then treated by 10% FBS at 37℃ for 30 minutes.

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