Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S15 antibody ( AP0083) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-p53-S15 antibody (AP0083). Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of U2OS cells using Phospho-p53-S15 antibody (AP0083). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-p53-S15 pAb (AP0083). Western blot was performed from the immunoprecipitate using Phospho-p53-S15 pAb (AP0083) at a dilition of 1:1000. 293T cells were treated by UV at room temperature for 30 minutes after serum-starvation overnight, and then treated by 10% FBS at 37℃ for 30 minutes.
|Product name||Phospho-p53-S15 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IF 1:50 - 1:200
IP 1:50 - 1:100
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence Immunoprecipitation|
|Cellular location||Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body|
(Adenocarcinoma human alveolar basal epithelial cells, A549)
WB(Mus musculus, Homo sapiens)
* For research use only. Not for therapeutic or diagnostic purposes.
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